Tirzepatide Purification Report (Method 2)
Primary Purification
Primary Chromatogram
Primary Purification Method
| Column | BS C18 Pro 10μm 120Å |
| Injection | 14g/L crude |
| Detection | 280nm / 254nm |
| Mobile Phase A | 50mM NH4Ac + 50mM NH4Cl pH6.5 |
| Mobile Phase B | Preparative ACN |
| Flow Rate | 3mL/min |
| Gradient | 0min:30%B → 55min:43%B |
Primary Purification Results
75.5% → 98.2%
Purity Improvement
80.1%
Yield
Secondary Purification
Secondary Chromatogram
Secondary Purification Method
| Column | BS C18 Pro 10μm 120Å |
| Injection | Primary purified fraction |
| Detection | 280nm / 254nm |
| Mobile Phase A | 20mM (NH4)2SO4 pH2.5 |
| Mobile Phase B | Preparative ACN |
| Flow Rate | 3mL/min |
| Gradient | 0min:35%B → 60min:45%B |
Secondary Purification Results
[Purity]
Final Purity
[Impurity]
Single Impurity
[Yield]
Yield
Conclusion
[Conclusion content will be updated here]