Semaglutide (Synthetic) Purification Chromatography Report
Primary Purification
Primary Chromatogram
Primary Purification Method
| Column | HSC8 10-100 |
| Injection | 14g/L |
| Detection | 210nm / 254nm |
| Mobile Phase | A: 3% HAC | B: ACN |
| Flow Rate | 60mL/min |
| Gradient | 0min:30%B → 100min:40%B |
Primary Purification Results
78% → 98.6%
Purity Improvement
90%
Yield
Secondary Purification
Secondary Chromatogram
Secondary Purification Method
| Column | BSC18 10-120 |
| Injection | 10g/L |
| Detection | 210nm / 254nm |
| Mobile Phase | A: 10mM NH4Ac | B: ACN |
| Flow Rate | 40mL/min |
| Gradient | 0min:25%B → 60min:35%B |
Secondary Purification Results
99.58%
Final Purity
0.09% / 0.08%
Front/End Impurities
85%
Yield
Conclusion
The two-step purification process delivers pharmaceutical-grade semaglutide:
Primary Purification
- 78% crude → 98.6% purity
- 90% process yield
- HSC8 column with HAC/ACN system
Secondary Purification
- Final purity 99.58%
- Impurities: 0.09% (front) / 0.08% (end)
- 85% yield
- BSC18 column with NH4Ac/ACN system
The optimized process achieves exceptional purity (>99.5%) with tight impurity control and combined yield of 76.5%.