Liraglutide Purification Chromatography Report
Chromatogram
Method Parameters
Preparative HPLC
| Column | CH C18 10-120 |
| Injection Concentration | 14g/L |
| Detection Wavelength | 280nm (Main) / 240nm (Monitor) |
| Mobile Phase A | 20mM Tris-HCl pH 8.5 |
| Mobile Phase B | Acetonitrile (ACN) |
| Flow Rate | 60mL/min |
| Gradient Program |
0min → 30% B 100min → 45% B
|
Purification Results
90% → >99%
Crude → Final Purity
<0.5%
Single Unknown Impurity
85%
Process Yield
Process Performance
| Stage | Purity | Impurities | Yield |
|---|---|---|---|
| Crude Material | 90% | Not specified | N/A |
| Primary Purification | >99% | <0.5% | 85% |
Conclusion
The developed purification process demonstrates excellent performance for liraglutide purification:
- High Purity Enhancement: 90% crude material purified to >99% final purity
- Stringent Impurity Control: Single unknown impurity maintained below 0.5% threshold
- Efficient Recovery: 85% process yield indicates robust method performance
The CH C18 10-120 column with Tris/ACN mobile phase system proves effective for industrial-scale GLP-1 analog production.