Retatrutide Purification Report
Primary Purification
Primary Chromatogram
Primary Purification Method
| Column | BS C18 10μm 120Å |
| Injection | 14g/L crude |
| Detection | 210nm / 254nm |
| Mobile Phase | A: 3% HAC | B: Preparative ACN |
| Flow Rate | 3mL/min |
| Gradient | 0min:30%B → 100min:45%B |
Primary Purification Results
82% → 98.54%
Purity Improvement
86.85%
Yield
0.5%
Max Single Impurity
Secondary Purification
Secondary Chromatogram
Secondary Purification Method
| Column | BS C18 10μm 120Å |
| Injection | Primary purified fraction |
| Detection | 280nm / 254nm |
| Mobile Phase A | 50mM NH4Ac + 50mM NH4Cl pH6.5 |
| Mobile Phase B | Preparative ACN |
| Flow Rate | 3mL/min |
| Gradient | 0min:30%B → 100min:40%B |
Secondary Purification Results
99.5%
Final Purity
0.25%
Max Single Impurity
89.9%
Yield
Conclusion
The two-step purification process achieves exceptional retatrutide quality:
Primary Purification
- 82% crude → 98.54% purity (+16.54% absolute improvement)
- 86.85% yield from crude material
- Maximum single impurity: 0.5%
- BS C18 column with HAC/ACN system
- 210nm primary detection wavelength
Secondary Purification
- Final purity 99.5% (ultra-pure grade)
- Maximum single impurity: 0.25% (50% reduction)
- 89.9% yield from primary fraction
- Ammonium acetate/chloride buffer system (pH6.5)
- 280nm primary detection for final QC
Overall Process Performance
Combined Yield: 78.08% (86.85% × 89.9%)
Total Purity Gain: 82% → 99.5% (+17.5%)
Impurity Reduction: From 18% → 0.5% → 0.25%
Note: The BS C18 column demonstrates excellent performance for both purification steps, with buffer system modification and wavelength optimization for final polishing. The process delivers ultra-pure retatrutide suitable for pharmaceutical applications.