Tirzepatide Purification Report
Primary Purification
Preparation Chromatogram
Purification Method
| Column | Sunpure-HS C8 Pro 10µm 100Å |
| Item Number | HST102000-0 |
| Sample | Tirzepatide crude |
| Loading Capacity | 1% |
| Flow Rate | 3mL/min |
| Mobile Phase | A: 0.1% TFA in water | B: 0.1% TFA in ACN |
Purification Results
99.73%
Purity
72.90%
Recovery
0.27%
Max Single Impurity
Conclusion
The single-step purification process achieves pharmaceutical-grade tirzepatide:
Key Results
- Final purity: 99.73% (exceeds pharmacopeial requirements)
- Recovery rate: 72.90% from crude material
- Maximum single impurity: 0.27%
- Loading capacity maintained at 1%
Product Characteristics
- Novel dual GIP/GLP-1 receptor agonist
- Binds albumin to prolong half-life
- Enhances both phases of insulin secretion
- Reduces glucagon levels glucose-dependently
- Promotes weight loss in T2DM patients
Performance Summary
Purity: 99.73% (HPLC)
Recovery: 72.90%
Impurity Profile: All <0.3%
Note: The Sunpure-HS C8 Pro material demonstrated excellent performance for tirzepatide purification, delivering high purity in a single chromatographic step. The method is robust and suitable for large-scale production of this dual agonist peptide therapeutic.