Optimizing Insulin Purification with Reversed-Phase Chromatography
Insulin, the vital protein hormone secreted by pancreatic beta cells in the islets of Langerhans, plays a crucial role in regulating blood sugar levels. Its production is stimulated by various substances including glucose, lactose, nucleic acids, arginine, and glucagon. As the only hormone capable of lowering blood glucose while promoting glycogen, fat, and protein synthesis, insulin remains the primary therapeutic agent for diabetes treatment.
Chromatogram Analysis
Figure 1: Insulin separation chromatogram using Sunpure-HS C8 packing material
Chromatography Specifications
| Packing material | Sunpure-HS C8 10μm 100Å |
| Item No. | HS8102000-0 |
| Sample | Insulin |
| Loading capacity | 1% |
| Flow rate | 3mL/min |
| Purity | 99.60% |
| Recovery | 88.50% |
Superior Performance in Insulin Purification
Our reversed-phase chromatography process utilizing the 10μm 100Å Sunpure-HS C8 packing material demonstrates exceptional performance in insulin purification. The results speak for themselves:
- High Purity: Achieving 99.6% purity ensures pharmaceutical-grade insulin suitable for therapeutic applications.
- Excellent Recovery: With 88.5% recovery, our process maximizes yield while maintaining quality.
- Consistent Performance: The Sunpure-HS C8 material provides reproducible results batch after batch.
Why Choose Our Chromatography Solution?
The Sunpure-HS C8 packing material is specifically engineered for biomolecule separation, offering:
Optimal Particle Size
10μm particles provide the ideal balance between resolution and flow characteristics for insulin purification.
Precise Pore Structure
100Å pore size ensures excellent accessibility for insulin molecules while maintaining structural integrity.
Surface Chemistry
C8 ligand chemistry provides the perfect hydrophobicity for insulin separation with minimal denaturation.
Ready to Optimize Your Insulin Purification Process?
Contact our chromatography specialists today to discuss how our Sunpure-HS C8 packing material can enhance your insulin production.
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